editado por Gianfranco Donelli. -- New York : Humana Press, 2014.

xiii, 380 p.; 26 cm. -- (Methods in molecular biology ; 1147)

ISBN 978-1-4939-0466-2.

Incluye referencias bibliográficas e índice.

Contenido: Investigations on biofilms in health and disease -- Methods for dynamic investigations of surface-attached in vitro bacterial and fungal biofilms -- Aqueous two-phase system technology for patterning bacterial communities and biofilms -- Quorum sensing in gram-positive bacteria: assay protocols for staphylococcal agr and enterococcal fsr systems -- Advanced techniques for In situ analysis of the biofilm matrix (Structure, composition, dynamics) by means of laser scanning microscopy -- Multiplex Fluorescence In Situ Hybridization (M-FISH) and Confocal Laser Scanning Microscopy (CLSM) to analyze multispecies oral biofilms -- Field emission scanning electron microscopy of biofilm-growing bacteria involved in nosocomial infections -- Experimental approaches to investigating the vaginal biofilm microbiome -- Imaging bacteria and biofilms on hardware and periprosthetic tissue in orthopedic infections -- Animal models to evaluate bacterial biofilm development -- Animal models to investigate fungal biofilm formation --Nonmammalian model systems to investigate fungal biofilms -- Investigations on anti-biofilm compounds and strategies -- Microbiological methods for target-oriented screening of biofilm inhibitors -- In vitro screening of antifungal compounds able to counteract biofilm development -- Biofilm matrix-degrading enzymes -- Efficacy evaluation of antimicrobial drug-releasing polymer matrices -- Antibiotic polymeric nanoparticles for biofilm-associated infection therapy -- Pharmacokinetics and pharmacodynamics of antibiotics in biofilm infections of Pseudomonas aeruginosa In vitro an In vivo -- Contribution of confocal laser scanning microscopy in deciphering biolfilm tridimensional structure and reactivity -- Chip calorimetry for evaluation of biofilm treatment with biocides, antibiotics, and biological agents -- Bacteriophage attack as an anti-biofilm strategy -- Photodynamic therapy as a novel antimicrobial strategy against biofilm-based nosocomial infections: study protocols -- Investigations on biofilms in the environment and manufacturing plants -- Capturing air-water interface biofilms for microscopy and molecular analysis -- Biofilm-growing bacteria involved in the corrosion of concrete wastewater pipes: protocols for comparative metagenomic analyses -- Culture-independent methods to study subaerial biofilm growing on biodeteriorated surfaces of stone cultural heritage and frescoes -- Biofilms of thermophilic bacilli isolated from dairy processing plants and efficacy of sanitizers.