editado por Donald Armstrong. -- Totowa, N.J. : Human Press, 2010.

xiv, 322 p.; 23 cm. -- (Methods in molecular biology ; 186)

ISBN 978-1-61737-222-3.

Incluye referencias bibliográficas e índice.

Contenido: Preface -- Techniques for free radical derived biomarkers -- Human xanthine oxidoreductase determination by a competitive ELISA -- Simultaneous determination of polyunsaturated fatty acids and corresponding monohydroperoxy and monohydroxy peroxidation products by HPCL -- Determination of products of lipid oxidation by infrared spectroscopy -- Detection of docosahexaenoic acid hydroperoxides in retina by gas chromatography/mass spectrometry -- Detection of lipid hydroperoxide-derived protein modification with polyclonal antibodies -- Techniques for determining the metabolic pathways of eicosanoids and for evaluating the rate-controlling enzymes -- Mass spectrometric quantification of F2-Isoprostanes as indicators of oxidative stress -- Formation of apolipoprotein AI-AII heterodimers by oxidation of high-density lipoprotein -- Detection of certain peroxynitrite-induced DNA modifications -- Hydroxyl and 1-hydroxyethyl radical detection by spin trapping and GC-MS -- Analysis of aliphatic amino acid alcohols in oxidized proteins -- Rapid determination of glutamate using HPLC technology -- A rapid method for the quantification of GSH and GSSG in biological samples -- Protein carbonyl measurement by ELISA -- N-(carboxymethyl) lysine (CML) as a biomarker of oxidative stress in long-lived tissue proteins -- Measurement of S-glutathionated hemoglobin in human erythrocytes by isoelectric focusing electrophoresis -- Oxidation of cellular DNA measured with the comet assay -- Measurement of DNA double-strand breaks with giant DNA and high molecular-weight DNA fragments by pulsed-field gel electrophoresis -- Evaluation of antibodies against oxygen free radicals-modified DNA by ELISA -- Techniques for antioxidant biomarkers -- Simultaneous analysis of multiple redox-active metabolites from biological matrices -- Determination of uric acid in urine by fast-scan voltammetry (FSV) using a highly activated carbon fiber electrode -- Measurement of tocopherol turnover in plasma and in lipoproteins using stable isotopes and gas chromatography/mass spectrometry -- Analysis of tocotrienols in different sample matrixes by HPLC -- Measurement of ß-carotene 15, 15 -dioxygenase activity by reverse-phase HPLC -- Ubiquinol/Ubiquinone ratio as a marker of oxidative stress -- Catechol- and pyrogallol-type flavonoids: analysis of tea catechins in plasma -- Pyruvate dehydrogenase complex as a marker of mitochondrial metabolism: inhibition by 4-hydroxy-2-nonenal -- Ceruloplasmin detection by SDS-PAGE, immunoblotting, and In situ oxidase activity -- Metallothionein determination by isocratic HPLC with fluorescence derivatization -- Quantification of oxidized metallothionein by a Cd-saturation method -- Fractionation of herbal medicine for identifying antioxidant activity -- Designing safer (soft) drugs by avoiding the formation of toxic and oxidative metabolites -- Statistical correction of the area under the ROC curve in the presence of random measurement error and applications to biomarkers of oxidative stress.